EVALUATION OF THE CONCENTRATION OF SHORT-CHAIN FATTY ACIDS IN THE ORAL FLUID IN PATIENTS WITH CHRONIC PERIODONTITIS

Abstract

To develop a method for preparing analysis samples by gas chromatography with mass-selective detection was the purpose of this work, which makes it possible to determine SCFA in oral fluid in healthy individuals and patients with chronic generalized periodontitis (CGP). The research included 18 patients with an average degree of chronic generalized periodontitis aged 28 to 61 years. An Agilent 5977B GC/MSD gas chromatograph with mass spectrometry an Agilent 8890 GC mass detector were used. HP-5MS Ultra inert 30 m × 250 mkm × 0.25 mkm columns were used. During the study of the oral fluid in patients with CGP using GC-MS, a change in the absolute content of short chain fatty acids was revealed. A decrease in the level of acetic acid to 0.26 ± 0.02 mg/g, propionic acid to 0.07 ± 0.006 mg/g, and butyric acid to 0.013 ± 0.002 mg/g was revealed in the oral fluid of patients with moderate CGP.