The objective of this study is to investigate the total phenol content and in vitro antioxidant potential of the different extracts of the fungal strain Ulocladium chartarum, isolated from Draceana leaf. The fungal culture was extracted with ethyl acetate (EtOAc) followed by methanol (MeOH). The antioxidant activities of the extracts were tested using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and phosphomolybdate complex assays, while total phenol content was evaluated by Folin- Ciocalteu method. The MeOH extract of Ulocladium chartarum displayed higher free radical scavenging activity (46.98% in DPPH assay) and antioxidant activity (28.86 mg ascorbic acid equivalent/g dried extract employing phosphomolybdate assay) in comparison to the EtOAc extract that displayed (11.63%) in DPPH assay and (9.5 mg ascorbic acid equivalent/g dried extract) in phosphomolybdate assay. It is noteworthy that a direct correlation between the total phenol content and the antioxidant activity was observed.